Research Paper Volume 16, Issue 1 pp 106—128

Jian-Pi-Yi-Shen formula alleviates renal fibrosis by restoring NAD+ biosynthesis in vivo and in vitro

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Figure 3. The effects of JPYSF on renal fibrosis in CKD mice. (A) Representative Western blot images of FN, Col-IV, α-SMA, and vimentin expression in the kidney of mice. (BE) Quantitative analysis of FN, Col-IV, α-SMA and vimentin normalized to α-Tubulin or GAPDH content (n = 6). (FJ) Representative immunohistochemical images and quantitative analysis of positive staining areas of FN, Col-IV, α-SMA and vimentin in the kidney of mice (n = 3). All images are shown at identical magnification, ×200, scale bar = 100 μm. Data are expressed as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001 between the indicated two groups).