Research Paper Volume 15, Issue 24 pp 15324—15339

Figure 4. PV interneurons were positively correlated with MMP-9 in the spinal cord of ALS mice with different onset time. The expression of PV and MMP-9 tested by immunofluorescence. The yellow boxes represent the typical positive cells (enlarged approximately twice on the original position). The typical PV and MMP-9 positive cells are enlarged approximately 4 times and shown on the top right panel. Scale bar = 100 μM (A). The expression of MMP-9 was tested by RT-qPCR (B) and western blot (C). Pearson’s correlation for protein levels of PV and MMP-9 in all animals (control and ALS) (D). ^***P < 0.001 vs. control at 90~100 days. ^###P < 0.001 vs. control at 130~140 days. In all experiments, half male and half female mice were used in each group (n = 6).