Research Paper Volume 15, Issue 23 pp 13608—13627

Figure 2. AGE improves eNOS activity in hyperlipidemic conditions. (A) Representative images of aorta surface stained with Oil Red O and atherosclerotic lesion area in the indicated groups. (B) Quantification of atherosclerotic lesion area (plaque area). (C) Serum NO levels in the indicated experimental groups. Data shown are relative to serum NO levels in control group. (D) Fluorescence microscopic detection of DAF-2DA staining for endothelial NO levels in the aorta and (E) its quantification. (F) Immunoblotting of eNOS and phosphorylated eNOS in aorta. (G) Quantitative analysis of phospho-eNOS based upon the eNOS expression. (H) Representative immunofluorescence images showing p-eNOS staining at Ser1177 (p-eNOS) in aortic sections. (I) Quantification of phosphorylation of eNOS at Ser1177. Values are presented as mean ± SEM (n = 4~9, ^#p < 0.05 vs. the NCD group; ^*p < 0.05 vs. the HFD group). Abbreviations: NCD: normal chow diet; HFD: high fat diet; AGE: Angelica gigas NAKAI extract.