Macrophage-specific deletion of Notch-1 induced M2 anti-inflammatory effect in atherosclerosis via activation of the PI3K-oxidative stress axis

Mingming Zhang; Xiangyong Yue; Xueping Zhao; Yonggang Lu; Hongtao Liu; Zhe Zhang; Huan Ma; Xing Wang; Hanying Xing

doi:doi:10.18632/aging.205342

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Research Paper Volume 15, Issue 24 pp 15196—15212

Macrophage-specific deletion of Notch-1 induced M2 anti-inflammatory effect in atherosclerosis via activation of the PI3K-oxidative stress axis

Figure 6. PI3K/AKT signaling pathway was involved in the Notch-1^MAC-KO-induced M2 polarization. (A) Western blotting showed that CD9, TSG101, Alix, CD206 and Arg-1 were significantly enhanced in peritoneal macrophages of Notch-1^MAC-KO mice, but iNOS and IL-6 were significantly reduced, which were reversed by LY-294002. Compared with W.T. group, the expression levels of CD9, TSG101, Alix and Arg-1 were enhanced in K.O. group; (B) statistical data for Western blot. ^*P < 0.05, ^**P > 0.01: Notch-1^MAC-KO vs. Notch-1^WT, Notch-1^WT vs. Notch-1^WT + LY-294002, Notch-1^MAC-KO vs. Notch-1^MAC-KO + LY-294002.