Research Paper Volume 15, Issue 24 pp 15196—15212
Macrophage-specific deletion of Notch-1 induced M2 anti-inflammatory effect in atherosclerosis via activation of the PI3K-oxidative stress axis
Figure 4. Correlation between PI3K and Notch-1 signaling activation. GSE155842 and GSE155745 datasets were downloaded from the GEO database, which contained AS samples. (A) The gene expression levels from GSE155842 were standardized using quartile division. (B) We identified 270 up-regulated differentially expressed genes (DEGs) and 436 down-regulated DEGs were obtained using criteria of |logFC| > 2, P adj < 0.05; (C) Based on GSE155842, the hierarchical clustering analysis exhibited a great difference in DEGs as indicated in the heat maps. (D) The survival analysis demonstrated that the survival time of patients with high expressions of Notch-1 was significantly shorter than those with low expressions. (E) The Notch-1 expression was also at a high level in the AS group. (F) Co-expression analysis revealed that Notch-1 was negatively associated with PI3K.