Research Paper Volume 15, Issue 24 pp 15183—15195

Figure 2. Effects of DMC-HA on the migration and invasion of glioma cells. (A) Cell viability in U87 and U251 cells was assessed using the CCK-8 assay following the indicated DMC-HA treatments. (B, C) Transwell assays were employed to evaluate the migration and invasion of U87 and U251 cells treated with 1 μM DMC-HA. (D) Western blot analysis was utilized to examine the expression of MMP-2 and MMP-9 proteins following a 48-hour treatment with DMC-HA and TGF-β1. (E) Western blot analysis was performed to assess the expression of ZO-1, N-cadherin, and Vimentin proteins after a 48-hour treatment with DMC-HA and TGF-β1. (F) Western blot analysis was used to detect the expression of Snail, Twist, and Slug proteins after a 48-hour treatment with DMC-HA and TGF-β1.