Research Paper Volume 15, Issue 24 pp 15183—15195

Figure 1. Effects of TGF-β1 on the EMT of glioma cells. (A) U87 and U251 cells were treated with the indicated concentrations of TGF-β1 (0, 2.5, 5 and 10 ng/mL) for 48 h, and then cell viability was detected using CCK-8 assay. (B) Effects of 10 ng/mL TGF-β1 on morphology of U87 and U251 cells. (C, D) U87 and U251 cells were treated with 10 ng/mL TGF-β1 for 48 h, and then the expression of ZO-1, N-cadherin and Vimentin proteins were detected by Western blot analyses. (E) Assessment of U87 and U251 cell invasion in response to 10 ng/mL TGF-β1 used a light microscope following Matrigel migration assay staining. (F) Assessment of U87 and U251 cell invasion in response to 10 ng/mL TGF-β1 used a light microscope following Matrigel invasion assay staining.