Research Paper Volume 15, Issue 22 pp 13558—13578

Figure 1. Identification and characterization of PTCHD4-AS. (A) Diagram of the screening strategy for conserved lncRNAs. (B) Relative expression levels of PTCHD4-AS in normal gastric epithelial cell line (GES-1) and GC cell lines (AGS, SGC7901, MGC803, MKN45 and NCI-N87). (C) Relative expression levels of PTCHD4-AS in paired GC tissues and adjacent normal gastric tissues. n=23. (D) Schematic diagram of the transcription of PTCHD4-AS. (E) Subcellular localization of PTCHD4-AS in SGC7901 and MGC803. DAPI labeled nuclei(blue); FISH probe labeled PTCHD4-AS (red), scale bar: 20 μm. (F) Relative expression of PTCHD4-AS after treatment of SGC7901 and MGC803 with the indicated concentrations of drugs for 48 h. Etop :20 μM, 5-FU :20 μM, CDDP: 10 μM, Dox:4 μM. All data are presented as mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.