Research Paper Volume 15, Issue 24 pp 14915—14929
hsa_circ_0002980 prevents proliferation, migration, invasion, and epithelial-mesenchymal transition of liver cancer cells through microRNA-1303/cell adhesion molecule 2 axis
Figure 4. circ_0002980 overexpression signally restrained LC cell proliferation through miR-1303. (A) The binding sites of circ_0002980 and miR-1303 were predicted using RegRNA software. (B) We applied dual luciferase reporter assays to analyze the changes in luciferase activity of WT- or Mut-circ_0002980. (C) RT-qPCR was utilized to identify miR-1303 expression after circ_0002980 overexpression. (D) HepG2 and Huh7 cells were then co-transfected with miR-1303 mimics and circ_0002980 overexpression plasmids, and the clone-forming ability was credited via clone formation assay. (E) After Edu dyeing, flow cytometer was utilized to confirm the proliferative potential in LC cells treated as D. *P < 0.05; **P < 0.01; ***P < 0.001.