Research Paper Volume 15, Issue 24 pp 14900—14914

Figure 2. Acetylshikonin promoted DNA fragmentation and cell cycle arrest in G2/M phase. (A, B) Flow cytometry image results indicating cell cycle progression in H1299 and A549 cells following treatment with acetylshikonin (0.5–10 μM) for 24 h and PI staining for 30 min (n = 4). (C, D) Western blot analysis showing CDK1 and cyclin B1 protein expression in H1299 and A549 cells treated with acetylshikonin (0.5–10 μM) for 6 h (n = 4). Untreated cells were used as controls. Results are shown as means ± SD. ^*p < 0.05 compared to untreated control.