Research Paper Volume 15, Issue 23 pp 14333—14371

Figure 6. Identification of the hub predictive genes to construct ADPS. (A) IHC staining images of five crucial aggrephagy genes. Panel1: DYNC1I2+ fibroblasts were marked with a red arrow. Protein was mainly expressed on cell membrane (source HPA. Patient ID: 448, female, age 76 years). Panel2: TUBA1A+ T cells were marked with a red arrow. Protein was mainly expressed on cytoplasmic/membranous (source HPA. Patient ID: 448, female, age 76 years). Panel3: TUBA4A+ T cells were marked with a red arrow. Protein was mainly expressed on cytoplasmic/membranous (source HPA. Patient ID: 2403, female, age 65 years). Panel4: UBA52+ T cells were marked with red arrows. Protein was mainly expressed in the nucleus (source HPA. Patient ID: 2393, female, age 54 years). Panel5: VIM + Macrophages were marked with red arrows. Protein was mainly expressed in the cytoplasmic/membranous (source HPA. Patient ID: 1421, female, age 76 years). (B–I) Kaplan-Meier survival curves of the ADPS regarding OS in the TCGA (B), GSE3141 (C), GSE31210 (D), GSE37745 (E), GSE50081 (F), GSE68465 (G), MetaGEO (H), and Meta cohorts (I). (J–Q) Time-dependent ROC curves of the ADPS regarding 1-, 3-, and 5-year OS in the TCGA (J), GSE3141 (K), GSE31210 (L), GSE37745 (M), GSE50081 (N), GSE68465 (O), MetaGEO (P), and Meta cohorts (Q).