Research Paper Volume 15, Issue 23 pp 14306—14322

Figure 5. Knockdown of DOK7 reverses the effect of miR-299-5p inhibitor on BLCA cells. (A) RT-qPCR detection of miR-299-5p levels in different groups (NC inhibitor, miR-299-5p inhibitor) of T24 and RT4 cells; (B) Cell proliferation in different groups (NC inhibitor + sh-NC, miR-299-5p inhibitor + sh-NC, or miR-299-5p inhibitor+sh-DOK7) of T24 and RT4 cells was examined by CCK-8 assay at 0, 24, 48, 72 hours; (C) Cell cycle detection in different groups of T24 and RT4 cells by flow cytometry; (D, E) Transwell migration (D) and invasion (E) assays in different groups of T24 and RT4 cells. 5 random fields of each sample were counted at 100X magnification, scale bar: 50 μm. ^**P < 0.01; ^***P < 0.001.