Research Paper Volume 15, Issue 23 pp 14306—14322

Figure 3. Knockdown of DOK7 promotes the proliferation and invasion of BLCA cells. (A) Validation of knockdown efficiency of DOK7 in UMUC3 and 5637 cell lines after the infection with lentivirus of sh-NC and sh-DOK7 (#1-3) by RT-qPCR. sh-DOK7 #1 showed the strongest silencing effect; (B) Cell proliferation of UMUC3 and 5637 cells with or without DOK7 knockdown was examined by CCK-8 assay at 0, 24, 48, 72 hours. DOK7 silencing promoted cell proliferation; (C) Cell cycle detection in different groups (sh-NC and sh-DOK7) of UMUC3 and 5637 cells by flow cytometry. DOK7 silencing promoted cell proliferation promoted cell cycle progression; (D, E) Transwell migration (D) and invasion (E) assays in UMUC3 and 5637 with or without DOK7 knockdown. 5 random fields of each sample were counted at 100X magnification. Scale bar: 50 μm. Enhanced migration and invasion were observed in the DOK7 silencing group. ^**P < 0.01; ^***P < 0.001.