Research Paper Volume 15, Issue 23 pp 14306—14322

Figure 2. Overexpression of DOK7 inhibits the proliferation and invasion of BLCA cells. (A) DOK7 protein levels in the T24 and RT4 cells with or without DOK7 overexpression detected by WB; (B) Cell proliferation of T24 and RT4 cells with or without DOK7 overexpression was examined by CCK-8 assay at 0, 24, 48, 72 hours. DOK7 overexpression suppressed cell proliferation; (C) EdU staining in assay T24 and RT4 cells with or without DOK7 overexpression. Blue: nucleus; Red: EdU staining. Reduced EdU staining signal was observed in the DOK7 overexpression group; (D) Cell cycle detection of different groups (vector and DOK7) of T24 and RT4 cells by flow cytometry. DOK7 overexpression caused the cell cycle arrest at G1/S transition; (E, F) Transwell migration (E) and invasion (F) assays in T24 and RT4 cells with or without DOK7 overexpression. 5 random fields of each sample were counted at 100X magnification. Scale bar: 50 μm. Reduced migration and invasion were observed in the DOK7 overexpression group. ^**P < 0.01; ^***P < 0.001.