Figure 2.Overexpression of DOK7 inhibits the proliferation and invasion of BLCA cells. (A) DOK7 protein levels in the T24 and RT4 cells with or without DOK7 overexpression detected by WB; (B) Cell proliferation of T24 and RT4 cells with or without DOK7 overexpression was examined by CCK-8 assay at 0, 24, 48, 72 hours. DOK7 overexpression suppressed cell proliferation; (C) EdU staining in assay T24 and RT4 cells with or without DOK7 overexpression. Blue: nucleus; Red: EdU staining. Reduced EdU staining signal was observed in the DOK7 overexpression group; (D) Cell cycle detection of different groups (vector and DOK7) of T24 and RT4 cells by flow cytometry. DOK7 overexpression caused the cell cycle arrest at G1/S transition; (E, F) Transwell migration (E) and invasion (F) assays in T24 and RT4 cells with or without DOK7 overexpression. 5 random fields of each sample were counted at 100X magnification. Scale bar: 50 μm. Reduced migration and invasion were observed in the DOK7 overexpression group. **P < 0.01; ***P < 0.001.
