Research Paper Volume 15, Issue 23 pp 14306—14322

Figure 1. DOK7 expression is suppressed in BLCA tumor and cell lines. (A) The mRNA expression levels of DOK7 in 108 pairs of BLCA cancer tissues (BLCA) and corresponding adjacent tissues (Normal) were detected by RT-qPCR method. DOK7 expression was significantly decreased in BLCA, P < 0.01; (B) The mRNA expression levels of DOK7 in BLCA tumor samples of different clinical stages (I+II and III+IV) were detected by RT-qPCR. DOK7 levels were significantly decreased in III+IV stage, P < 0.01; (C) The expression levels of DOK7 in BLCA tumor samples of different histological grades (Low and High) were detected by qRT-PCR. DOK7 levels were significantly decreased in high grade tissues, P < 0.001; (D) The expression levels of DOK7 in different clinical stages of TCGA-BLCA cancer tissues were analyzed by GEPIA. DOK7 levels were gradually decreased with the advancement of the clinical stage, P < 0.001; (E) Kaplan–Meier (KM) curve analysis of the overall survival BLCA patients in DOK7 low-expression group (n = 54) and high-expression group (n = 54). The overall survival rate of the low-expression group was significantly lower than that of the high-expression group, P < 0.001; (F) GEPIA online analysis of the TCGA BLCA cohort revealed the poor overall survival in BLCA patients with the low expression of DOK7; (G) K-M plotter online analysis found that the overall survival of BLCA patients with low expression of DOK7 was poor; (H) IHC staining of DOK7 protein expression levels in 3 pairs of cancer tissues and adjacent normal tissues under the magnification of 200X. DOK7 expression was significantly reduced in BLCA tumor tissues. The DOK7 staining was seen as dark signal in the nuclei, scale bar: 200 μm; (I) The protein levels of DOK7 in 6 pairs of BLCA cancer tissues and normal tissues were detected by WB. DOK7 protein levels were decreased in BLCA cancer tissues; (J) DOK7 mRNA expression levels in BLCA cell lines (RT4, T24, and UMUC3, 5637) and normal bladder cell line (SV-HU-1) were detected by RT-qPCR; (K) WB detection of DOK7 protein expression levels in BLCA cell lines and normal bladder cell line. ^**P < 0.01; ^***P < 0.001.