Research Paper Volume 15, Issue 24 pp 14791—14802

Figure 2. IGFBPL1 increases macrophage cholesterol efflux through ABCG1. (A) Relative mRNA level of genes relative to cholesterol uptake and efflux (n=3). (B) WB was used to detect the protein level of ABCG1. (C) Quantitative statistics on WB results by ImageJ (n=3). (D) Representative images of NBD cholesterol fluorescence staining of macrophages. (E) Quantitative results of average fluorescence intensity of per THP-1 macrophage cell (n=3). PA: palmitic acid, THP-1 macrophages were incubated with 100 μM PA for 48 h. PA + ov-IGFBPL1, THP-1 was transfected with over-expression plasmid of IGFBPL1 for 24 h and then incubated with 100 μM PA for 48 h. P<0.05 represents a significant statistical difference; Scale indicates 25 μm.