Research Paper Volume 15, Issue 24 pp 14791—14802

Figure 1. IGFBPL1 inhibits PA-induced lipid accumulation in macrophages. (A) Different expression gene in control samples and unstable atherosclerosis plaque human. Normal: the abdominal aortas intima of three patients without atherosclerosis. Atherosclerosis: aortic plaques of patients diagnosed with grade V or VI atherosclerosis. (B) Relative IGFBPL1 protein level (n=3). (C) Relative mRNA level of IGFBPL1 in PA or ox-LDL treated THP-1 macrophages (n=3). (D) Relative protein level of IGFBPL1 (n=3). (E) Relative IGFBPL1 mRNA level (n=3). (F) Representative images of oil red O staining. PA: palmitic acid, THP-1 macrophages were incubated with 100 μM PA for 48 h. ox-LDL: oxidized low density lipoprotein, THP-1 macrophages were treated with 100 μg/mL ox-LDL for 72 h. PA + ov-IGFBPL1, THP-1 was transfected with over-expression plasmid of IGFBPL1 for 24 h and then incubated with 100 μM PA for 48 h. P<0.05 represents a significant statistical difference; Scale indicates 25 μm.