Research Paper Volume 16, Issue 1 pp 1—14

Figure 4. Resveratrol increases in vitro rhythmic gene mRNA expression of core clock components in human adipose-derived progenitor cells (APCs) from older participants. Time course analysis of the in vitro rhythmic gene expression profiles in APCs from older participants after RSV treatment (blue) vs. control conditions (black). APCs were synchronized with 30% fetal bovine serum (FBS) for two hrs and treated with vehicle for 12 hrs. RNA was extracted from APCs, harvested every six hrs from 12 to 60 hrs post-synchronization. Abundance of transcripts was quantified by RT-PCR and normalized to Peptidylprolyl isomerase A (PPIA). Data are presented as mean ± SEM. When ARSER analysis detected a significant rhythm (P<0.05), symbols in the graphics represent oscillation in control (black), in RSV (blue), or in both groups. The effect of time, age and interactions was analyzed by linear mixed model. a: P<0.05 effect of time; b: P<0.05 effect of age; c: P<0.05 time by age interaction. * indicates significant differences using post-hoc comparisons. BMAL1: brain and muscle ARNT-like protein-1, CLOCK: circadian locomotor output cycles kaput; PER1/2: period 1/2, CRY1/2: cytochrome 1/2, D site of albumin promoter (albumin D-box) binding protein, RORα: RAR related orphan receptor A; NR1D1/2, nuclear receptor subfamily 1 group D member 1/2.