Research Paper Volume 15, Issue 23 pp 14086—14108

Figure 1. RNA sequencing of amniocytes derived from the normal and trisomy 21 groups. (A) Karyotyping results of cultured amniocytes from the trisomy 21 and normal groups, including males and females. Black arrow indicates the additional Chr21. (B) Gestational age of pregnant women in this study (nNormal = 5, nTrisomy = 10). (C) PCA plot comparing the normal and trisomy 21 groups according to their mRNA expression using counts per million (CPM) normalization. (D) Pearson correction heatmap of different samples in the normal and trisomy 21 groups. (E) Average mRNA expression per chromosome was calculated and quantified in the normal and trisomy 21 groups (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, two-tailed t test).