Research Paper Volume 15, Issue 23 pp 14019—14038
Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats
Figure 4. STAT3 induces transcriptional activation of IGF1 in rADSCs. (A) The dose-dependent inhibition of STAT3 for 24 h in rADSCs. (B) Knockdown of STAT3 by transfection of shSTAT3 for 24 h and its expression levels by immunoblot analysis. (C, D) The mRNA expression levels of STAT3 and IGF1 were determined by qRT PCR analysis. (E) In silico study illustrated the interaction between STAT3 and IGF1 promoter levels. (F) Transfection with PGL4-IGF1 promoter plasmid and silencing STAT3 expression with shSTAT3 for 24 h and further analysis by luciferase activity. (N = 3; *p < 0.05; **p < 0.01; ***p < 0.001 indicate significant differences).