Research Paper Volume 15, Issue 23 pp 14019—14038
Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats
Figure 1. Regulation of growth factors secretion (IGF1 & IGFBP3) in rADSCs. (A) Effects of co-chaperone CHIP overexpression on IGF1 secretion against Ang II challenge for 24 h in rADSCs by immunoblot analysis. (B) Level of secreted proteins such as IGF1 and IGFBP3 was estimated by Western blot after 24 h transfection of HA-vector and HA-CHIP wild-type in rADSCs. (C) Expression levels of IGF1R and its downstream signaling proteins after overexpression with either HA-vector or HA-CHIP wild-type for 24 h were analyzed by Western blotting. (N = 3; *p < 0.05; **p < 0.01 indicate significant differences).