Research Paper Volume 15, Issue 24 pp 14749—14763

Figure 6. IGF1 treatment attenuated miR-184-mediated renal cell damage. CaOx (100 μg/mL) was used to treat HK2 cells transfected with miR-184 mimics and IGF1 (100ng/ml) treatment for 24 hours. (A) qRT-PCR was used to examine the profiles of miR-184 and IGF1R. (B) CCK8 assay was used to check cell viability. (C) TUNEL staining monitored cell apoptosis. Scale bar=100 μm. (D) Western blot analysis was used to determine the profiles of apoptosis-related proteins. (E, F) Western blot analysis was used to determine the profiles of the NF-κB and Nrf2/HO-1 pathways. (G) ELISA revealed the levels of the inflammatory cytokines IL-6, TNF-α, MCP1, and ICAM1. (H) Immunofluorescence evaluated the activity of ROS. Scale bar=100 μm. *P<0.05, **P<0.01, ***P<0.001 (vs. CaOx+miR-NC). ^^P<0.01, ^^^P<0.001 (vs. CaOx+miR-184). N=3.