Research Paper Volume 15, Issue 24 pp 14749—14763
Downregulating miR-184 relieves calcium oxalate crystal-mediated renal cell damage via activating the Rap1 signaling pathway
Figure 5. miR-184 downregulation activated the Rap1 pathway. miR-NC and miR-184 were used to transfect HK2 cells. miR-in and miR-184-in were used to transfect HPC cells with CaOx (100 μg/mL) for 24 hours of treatment. (A) Western blot analysis was used to determine the protein profiles of p-IGF1R, IGF1R, p-PI3K, PI3K, Akt, and p-Akt in the Rap1 pathway. (B) Fluorescence was conducted for evaluating IGF1R expression. Scale bar=100 μm. (C) Western blot analysis was used to determine the protein profiles of p-IGF1R, IGF1R, p-PI3K, PI3K, Akt, and p-Akt in the Rap1 pathway. **P<0.01, ***P<0.001 (vs. Con). ^^P<0.01, ^^^P<0.001 (vs. CaOx+miR-in). N=3.