Research Paper Volume 15, Issue 24 pp 14749—14763
Downregulating miR-184 relieves calcium oxalate crystal-mediated renal cell damage via activating the Rap1 signaling pathway
Figure 1. miR-184 expression features in the kidney stones model. (A) CaOx (100 μg/mL) was applied to treat HK2 cells for 24 hours. The top 10 DE miRNAs were determined by RT-PCR. (B) The rat kidney stones model was induced through the intraperitoneal injection of glyoxylate (80 mg/kg/d; 200 μL). qRT-PCR examined miR-184 expression in the kidney tissues of the rats. ***P<0.001 (vs. Normal). N=10. (C, D) CaOx (5, 10, 25, 50, 100, 200 μg/mL) was applied to treat HK2 cells and HPCs for 24 hours. qRT-PCR analysis of miR-184 expression in HK2 and HPC cells. (E, F) CaOx (100 μg/mL) was applied to treat HK2 cells and HPCs for 3, 6, 12, 24, 48 hours. qRT-PCR analysis of miR-184 expression in HK2 and HPC cells. NSP>0.05, *P<0.05, **P<0.01, ***P<0.001 (vs. control). N=3.