Research Paper Volume 15, Issue 23 pp 13980—13997

Figure 3. The effect of overexpression of GPRC5D-AS1 on muscle regulatory factors. (A) qRT-PCR analyzed gene expression of Myf5, MyoG, MyoD and Mef2c. HSMM was control group. Dex (15 mM) was added in HSMM to establish atrophy cell model (model group). Empty plasmid (NC group) and GPRC5D-AS1-OE plasmid (lncRNA-OE group) were transfected into atrophy cell model and incubated for 48 h. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group. (B) Protein expression of Myf5, MyoG, MyoD and Mef2c detected by Western blot. Groups were set as previously mentioned. Empty plasmid and GPRC5D-AS1-OE plasmid were transfected into atrophy cell model and incubated for 48 h. (C) Quantitative analysis of western blot. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group.