Research Paper Volume 15, Issue 23 pp 13980—13997

Figure 2. The effect of overexpression of GPRC5D-AS1 on cell viability and cell apoptosis. (A) Cell viability was assessed by CCK-8 assay. HSMM was control group. Dex (15 mM) was added in HSMM to establish atrophy cell model (model group). Empty plasmid (NC group) and GPRC5D-AS1-OE plasmid (lncRNA-OE group) were transfected into atrophy cell model and incubated for 24 h, 48 h and 72 h. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group. (B) Cell apoptosis was assessed by flow cytometry. Groups were set as previously mentioned. Empty plasmid and GPRC5D-AS1-OE plasmid were transfected into atrophy cell model and incubated for 48 h. (C) Quantitative analysis of cell apoptosis. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group.