Research Paper Volume 15, Issue 23 pp 13980—13997

Figure 1. GPRC5D-AS1 restored the proliferation level of atrophic myoblasts. (A) FISH assay was utilized to identify the subcellular localization of long non-coding RNA (lncRNA) GPRC5D-AS1 in cells (200 ×). The red fluorescence represents GPRC5D-AS1, and the blue fluorescence represents the cell nucleus. Quantification of fluorescence intensities (Gray Value) by ImageJ software. (B) The efficiency of overexpression vector encoding GPRC5D-AS1 was examined by qRT-PCR. Human skeletal muscle myoblasts (HSMM) were the control group. 15 mM Dexamethasone (Dex) was added in HSMM to establish atrophy cell model (model group). Empty plasmid (NC group) and GPRC5D-AS1-OE plasmid (lncRNA-OE group) were transfected into atrophy cell model. Differences among groups were analyzed using ANOVA with Bonferroni’s multiple comparison test. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group. (C) Effects of GPRC5D-AS1 overexpression on cell cycle progression using flow cytometry after propidium iodide staining. Representative images were shown. ^*P < 0.05, ^**P < 0.01 compared with control group; ^#P < 0.05, ^##P < 0.01 compared with model group; ^&P < 0.05, ^&&P < 0.01 compared with NC group.