Research Paper Volume 15, Issue 23 pp 13961—13979

Figure 6. CTSV antibody blockade inhibits adhesion, migration, and invasion in lung cancer cells. (A) The schematic diagram for the experimental procedures of generating CTSV antibodies. (B) A549 cells treated with CTSV antibody were then subjected to immunoblotting assay using anti-fibronectin, anti-E-cadherin, and anti-N-cadherin antibodies. The grouping of blots cropped from different parts of the same gels. Full-length blots are shown in Supplementary Figure 1. (C–E) Adhesion assays were performed for the indicated stable A549, NCI-H1975, and NCI-292 cells pre-treated with CTSV antibody. (F–K) The indicated stable A549, NCI-H1975, and NCI-H292 cells were treated with CTSV antibody and examined by migration and invasion assay. The representative data are repeated for three biologically independent experiments. Student’s t-tests were used for data analysis; ^**P-value < 0.01, ^***P-value < 0.001, and ^****P-value < 0.0001.