Research Paper Volume 15, Issue 23 pp 13920—13943

Figure 6. The mutual regulatory relationship between hsa-miR-300 and TP53. (A) A transcription factor-gene-miRNA network centered on hsa-miR-300. Arrows indicate regulatory relationships. (B–D) The ability of expression and interference vectors to regulate TP53 expression in A375 cells from mRNA and protein levels, respectively. (E) In A375 cells, TP53 vector affected the expression of hsa-miR-300. (F) Schematic diagram of miR-300 regulating site in TP53 UTR. “UTR-M” represents the mutation site and “UTR-D” represents the deletion site. (G) In 293T cell line, dual-luciferase reporting assay confirmed that hsa-miR-300 regulates TP53. (H–J) In A375 cells, hsa-miR-300 regulates the expression level of TP53. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.