Research Paper Volume 15, Issue 24 pp 14720—14732
Meteorin-like protein elevation post-exercise improved vascular inflammation among coronary artery disease patients by downregulating NLRP3 inflammasome activity
Figure 4. Metrnl alleviated palmitic acid (PA)-induced metabolic dysfunction among human umbilical cord vein endothelial cells (HUVECs) by increasing aerobic respiration and reducing oxidative stress. (A) Reactive oxygen species (ROS) levels among control (Con), PA-treated (PA), and co-treated PA+Metrnl (PA+Metrnl) HUVECs. (B) Representative traces of oxygen consumption rate (OCR) measurements for the 3 HUVEC groups through different ports in the flux analyzer: oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and rotenone. (C) Quantification of basal, ATP-linked, and maximal respiration rates, derived from OCR measurements, for all 3 groups. (D) Representative traces of extracellular acidification rate (ECAR) measurements through different ports in the flux analyzer: glucose, oligomycin and 2-deoxy-glucose (2-DG). (E) Quantification analysis of glycolysis and glycolytic capacity, derived from ECAR measurements, for all 3 groups. Results are shown as mean ± SD. N =5/group. * P<0.05 was statistically significant.