Research Paper Volume 15, Issue 23 pp 13738—13752
Involvement of RFC3 in tamoxifen resistance in ER-positive breast cancer through the cell cycle
Figure 8. Effect of RFC3 knockdown on MCF-7R cells. (A) qPCR assay showed that RFC3 mRNA expression was significantly reduced in MCF-7R cells with knockdown of RFC3 compared to the control group. (B) WB assay showed that RFC3 protein expression was significantly reduced in MCF-7R cells with RFC3 knockdown compared to control cells. (C) CCK-8 assay showed that the proliferation rate of RFC3-knockdown MCF-7R cells treated with TAM was significantly reduced compared with the control group. (D, E) Flow cytometry apoptosis assay showed that apoptosis was significantly increased in MCF-7R cells with RFC3 knockdown after TAM treatment compared to the control group.