Research Paper Volume 15, Issue 22 pp 13452—13470

Figure 1. Fer-1 alleviated TMJOA progression in a rat UAC model. (A) Schematic model of the time course for establishment of the unilateral anterior crossbite (UAC) model of TMJOA rat treated with Fer-1 (1 mg/kg) by articular injection. (B) Representative H&E and Safranin O/fast green staining of control and UAC-induced TMJOA rat treated with/without Fer-1. Scale bars, 200 μm. (C) Osteoarthritis Research Society International (OARSI) score evaluated based on Safranin O/fast green staining. n = 3. (D) Representative micro-CT of control and UAC-induced TMJOA rat treated with/without Fer-1. (E) Quantitative analysis of subchondral bone parameters of control and UAC-induced TMJOA rat treated with/without Fer-1. (F) Representative immunofluorescence staining of Col2α1, MMP-13 and GPX-4 of control and UAC-induced TMJOA rat treated with/without Fer-1. Arrow heads indicated positive cells. n = 3. Scale bars, 50 μm. (G) Quantitative analysis of immunofluorescence staining of Col2α1, MMP-13 and GPX-4 of control and UAC-induced TMJOA rat treated with/without Fer-1. (All quantified data are shown as mean ± SEM; #p < 0.05, ##p < 0.01, *p < 0.05, **p < 0.01, by one-way ANOVA followed by the Tukey-Kramer test, #: CON vs UAC, *: UAC vs UAC+Fer-1).