Research Paper Volume 15, Issue 23 pp 13693—13709

Figure 6. Glycitin antagonized the activation of the NF-κB signaling pathway. (A) The NF-κB1 level was assayed via RT-PCR. (B–D) Protein levels of pIκBα and p-P65 were tested by WB method using image J. (E, F) Immunofluorescence to detect pIκBα in HNPCs of each indicated group (n=3). Scale bar, 100 μm. (G, H) The HNPCs were treated with or without Glycitin (100 μg/ml) for 12 hours, and then adding TNF-α (10 ng/mL) for 6 h. Immunofluorescence cell staining was applied to determine the p65 location. Scale bar: 100 μm. (I–K) Nuclear translocation of p65 were determined by WB analysis using ImageJ. The values represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.