Research Paper Volume 15, Issue 23 pp 13693—13709
The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells
Figure 3. Glycitin alleviated the TNF-α-induced HNPCs apoptosis. (A–C) Transcriptional and (D) protein levels of Bcl-2, Bax and Cleaved Caspase-3 in human primary nucleus pulposus cells as determined by RT-PCR and WB method. (E–G) Quantitative analysis of immunoblotting in (D), assayed by ImageJ program. (H, I) Quantification of NP cell apoptosis by flow cytometry. (J) TUNEL staining of the human NP cells of every group (n=3). Nuclei were stained with DAPI. Scale bar, 100 μm. The values shown represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.