Research Paper Volume 15, Issue 23 pp 13693—13709
The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells
Figure 2. Glycitin restrained ECM metabolism and alleviated. (A–D) Transcriptional and (E) protein levels of Aggrecan, Col-2, ADAMTs-5 and MMP-13 in cells as determined by RT-PCR and WB method. (F–I) Quantitative analysis of immunoblotting in (E), assayed by ImageJ program. (J–M) IF staining of COX-2, fluorescence intensity analysis was performed using ImageJ program. Scale bar: 100 μm. The values represent the mean ± SD of three independent experiments. *p < 0.05 vs. control group.