Research Paper Volume 15, Issue 22 pp 12738—12748
Contribution of membrane raft redox signalling to visfatin-induced inflammasome activation and podocyte injury
Figure 1. Effects of visfatin on aggregation of gp91phox, p47phox and MR clusters, O2·− production in podocytes. (A) Representative confocal microscopic images of MR clusters (Green) and aggregation of gp91phox (Red) and MR clusters and p47phox (Red) in podocytes (original magnification, 400x). Only overlaid images were presented. Yellow spots in overlaid images indicate co-localization of the MR and gp91phox or p47 phox. (B) Summarized data showing the effects of Visfatin, MR disruptor, MCD and NADPH oxidase inhibitors DPI on MR- gp91phox or MR-p47clusters (n = 6). Values are means ± SEM, showing fold changes as compared with control. (C) Summarized data showing the effects of visfatin on O2·− production (n = 5 − 7). Values are means ± SEM, showing fold changes as compared with control. Abbreviations: Ctrl: Control; Vis: Visfatin; DPI: diphenyleneiodonium; MCD: methyl-β-cyclodextrin. *P < 0.05 vs. control; #P < 0.05 vs. visfatin.