Research Paper Volume 15, Issue 22 pp 13194—13212
Electroacupuncture ameliorates AOM/DSS-induced mice colorectal cancer by inhibiting inflammation and promoting autophagy via the SIRT1/miR-215/Atg14 axis
Figure 6. SIRT1 promotes autophagy via the miR-215/Atg14 axis. (A) Detection of the expression level of Atg14 mRNA following overexpression of SW480 and HCT116 and inhibition of SIRT1. (B–D) Detection of the expression level of autophagy-related molecules mRNA following overexpression of SW480 and HCT116 and inhibition of SIRT1. (E) Detection of the expression level of autophagy-related molecular proteins following overexpression and inhibition of SIRT1 in SW480 and HCT116. (F) Detection of the expression of Atg14 and other autophagy-related molecules following overexpression of SIRT1, and insertion of miR-215 mimics in SW480 and HCT116. Three mice were analyzed in each group. Data are presented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the control group.