Research Paper Volume 15, Issue 22 pp 12749—12762

Figure 6. NecB suppressed age-dependent increase in vacuole area in D. melanogaster’s brain. (A) Oregon-RC males, (B) Oregon-RC females, (C) DGRP-100 males and (D) DGRP-100 females. Ctrl represents standard cornmeal medium; Rap-50 represents cornmeal medium supplemented with Rapamycin at 50 μg/mL; Rap-200 represents cornmeal medium supplemented with Rapamycin at 200 μg/mL; NecB-50 represents cornmeal medium supplemented with NecB at 50 μg/mL; and NecB-200 represents cornmeal medium supplemented with NecB at 200 μg/mL (Supplementary Table 1). The quantification of the neurodegeneration and vacuolar lesions based on the histological analysis of the Drosophila brains were observed at the 90th days post-eclosion. The data are from three independent experiments, and values are shown as mean ± s.e.m. Statistical significance was analyzed with an unpaired Student’s t-test and indicated as ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 from three independent experiments (n = 30).