Research Paper Volume 15, Issue 22 pp 12749—12762

Figure 1. NecB increased the lifespan of Drosophila melanogaster. (A) Oregon-RC males, (B) Oregon-RC females, (C) DGRP-100 males and (D) DGRP-100 females. Ctrl represents standard cornmeal medium; Rap-50 represents cornmeal medium supplemented with Rapamycin at 50 μg/mL; Rap-200 represents cornmeal medium supplemented with Rapamycin at 200 μg/mL; NecB-50 represents cornmeal medium supplemented with NecB at 50 μg/mL; and NecB-200 represents cornmeal medium supplemented with NecB at 200 μg/mL (Supplementary Table 1). For the lifespan assay, the survival rate of 150 flies from each group was monitored with medium change every 2 days. Comparisons were made using log-rank tests. The p values (log-rank tests) for each strain and each sex were as follows. (A) Oregon-RC male flies: Ctrl versus RAP-50 (p = 0.004), RAP-200 (p < 0.0001), NecB-50 (p < 0.0001), and NecB-200 (p < 0.0001), respectively. (B) Oregon-RC female flies: Ctrl versus Rap-50 (p = 0.0015), Rap-200 (p < 0.0001), NecB-50 (p < 0.0001), and NecB-200 (p < 0.0001), respectively. (C) DGRP-100 male flies: Ctrl versus RAP-50 (p = 0.0006), Rap-200 (p < 0.0001), NecB-50 (p < 0.0001), and NecB-200 (p < 0.0001), respectively. (D) DGRP-100 female flies: CTRL versus Rap-50 (p < 0.0001), Rap-200 (p < 0.0001), NecB-50 (p < 0.0001), and NecB-200 (p < 0.0001), respectively. The percentage of surviving flies is shown along with the maximum lifespan in each group (n = 150).