Research Paper Volume 15, Issue 22 pp 13163—13175

Figure 3. Hypoxia promotes exosome secretion from chondrosarcoma cells that induce M2 macrophage expression arginase-1 protein. (A) Macrophages were treated with SW1353-N-exo, SW1353-H-exo, or control (PBS). After 48 h, macrophage markers, including intracellular markers and cytokines (arginase-1, TGF-β, IL-10, IL-1β, iNOS), were detected by qRT-PCR. (B, C) Western blot analysis was used to detect the expression of the M2 intracellular marker arginase-1. All replicate number ≧ 3. ^#p < 0.05 compared with the PBS group; ^*p < 0.05 compared with the SW1353-N-exo group.