Research Paper Volume 15, Issue 21 pp 11764—11781

Figure 5. The double combination, doxycycline plus azithromycin, and a triple combination, doxycycline plus azithromycin plus vitamin C, were effective in decreasing the ATP levels in C. elegans. (A) Schematic representation of the protocol for CellTiter-Glo luminescent assay performed in experiments using the double and triple combination. (B) Measurement of ATP levels. The final concentrations of the compounds used in this experiment were 1 μM for doxycycline and azithromycin, and 250 μM for vitamin C. Data are shown as the mean ± SEM; Statistical analysis was performed using one-sample t-test. ^*p < 0.05, ^**p < 0.01. Experiments were repeated 4 times, using 5 worms for each condition.