Research Paper Volume 15, Issue 23 pp 13680—13692

Figure 3. Effects of APN on TXNIP expression and its interaction with NLRP3. (A) TXNIP expression was assayed by qRT-PCR and western blot in normal and degenerated human NP tissue samples (n=10). (B, C) After 12 h of treatment with PBS or LPS, human NP cells were transfected with or without lentiviral vector expressing APN for 24 h (n=3). (B) TXNIP expression was evaluated by qRT-PCR and western blot. (C) Co-IP analysis of TXNIP interaction with NLRP3. All results are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.