Research Paper Volume 15, Issue 22 pp 13010—13040

Figure 9. Hippo-YAP signal pathway mediates TOP2A-driven LIHC cell growth, migration, and invasion. (A) The expression of TOP2A was detected by qRT-PCR and Western blot in normal liver cell line and four liver cancer cell lines. (B) The knockdown efficiency of TOP2A siRNA was determined by qRT-PCR and Western blot in HCCLM3 and Hep3B cells. (C) The expression level of p-YAP and YAP was detected by Western blot in the indicated groups of HCCLM3 and Hep3B cells. β-actin was used as a control. (D, E) the effect of TOP2A on cell proliferation was assessed by colony formation and CCK8 assays in HCCLM3 and Hep3B cells. (F) The effect of the TOP2A on cell migration and invasion was tested by transwell assays in HCCLM3 and Hep3B cells. *P < 0.05, ***P < 0.001, and ****P < 0.0001. Data presented as mean ± standard deviation (SD). Experiments were repeated at least three times.