Research Paper Volume 15, Issue 22 pp 12998—13009

Figure 2. circFoxp1 regulates HSF proliferation, migration, apoptosis, and ECM deposition in vitro. (A) qRT-PCR analysis of overexpression efficiency of circFoxp1 in HSFs transfected with OE-NC or OE-Foxp1 lentivirus. (B) CCK-8 assay of the proliferation ability in HKFs transfected with OE-NC or OE-circFoxp1. (C) Colony formation assays for HSF cells with circFoxp1 overexpression. (D) Transwell assays showing overexpression of circFoxp1 obviously enhanced cell migration in HSF compared with OE-NC cells. (E) Transwell assays showing overexpression of circFoxp1 obviously enhanced cell invasion in HSF compared with OE-NC cells. (F) Cell apoptosis analyses via flow cytometric assays in OE-circFoxp1-transfected HSF cells. n = 3 biologically independent samples. Data shown are mean ± SD, ^*p < 0.05, ^**p < 0.001.