Research Paper Volume 15, Issue 22 pp 12998—13009

Figure 1. Expression levels and correlation analysis of has-circFoxp1 and linear Foxp1 mRNA in keloid tissues. (A) qRT-PCR analysis of relative RNA levels of circFoxp1 in keloid tissues and normal skin. (B) qRT-PCR analysis of relative RNA levels of Foxp1 in keloid tissues and normal skin. (C) FISH assay indicting the cellular location of circFoxp1 (red) in the HSFs (magnification 400× and scale bar 20 μm). Probe sequence: TGACACGGGAACTTTAGAAATGAT. (D) RNAscope assay indicating the cellular location of circFoxp1 (red) in Keloid tissues (magnification 100× and scale bar 100 μm). Most of the expression of circFoxp1 was located in the cytoplasm. (E) Map of circFoxp1 derived from the 8-12 exon region of the FOXP1 gene. (F) Sanger sequencing results indicating the cyclization site of circFoxp1 (arrow). ^**p < 0.001.