Research Paper Volume 15, Issue 22 pp 12966—12981

Figure 4. Effects of hsa-mir-503/hsa-mir-1247 knockdown on target mRNAs expression and 22RV1 cells apoptosis. (A, B) The mRNA and protein levels of FGF2, DUSP19, and SLC2A5 were significantly increased after hsa-mir-503 knockdown. ^*p < 0.05, ^**p < 0.01. (C) The binding of hsa-mir-503 to FGF2-3’-UTR verified by dual luciferase reporter assay (^**p < 0.01). (D, E) The mRNA and protein levels of FGF2 and VSTM4 were significantly decreased after hsa-mir-1247 knockdown. (F) The binding of hsa-mir-1247 to FGF2-3’-UTR verified by dual luciferase reporter assay (^**p < 0.01). (G) The apoptotic rate of 22RV1 cells was significantly increased after hsa-mir-503 knockdown. (H) The apoptotic rate of 22RV1 cells was not significantly changed after hsa-mir-503 knockdown (^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. NC inhibitor).