Figure 1. KIFC1 was overexpressed in tumor tissues and facilitates ESCC Proliferation. (A) Immunohistochemical staining is employed to detect KIFC1 protein expression in both healthy and ESCC tissues; (B) The A.H. score is used to statistically analyze KIFC1 protein expression in healthy, adjacent noncancerous, and tumor tissues of the esophagus; (C) Analysis of KIFC1 protein expression in adjacent non-tumor esophageal tissues and their paired ESCC tissues is conducted; (D) Knock down of KIFC1 using shRNA; (E) The influence on clonal formation ability due to knockdown of KIFC1 expression is assessed; (F) In vivo analysis of the proliferative ability associated with KIFC1 is performed. n = 30, **< 0.01.