Research Paper Volume 15, Issue 22 pp 12852—12872

Figure 7. Effects of the candidate peptide Acg2-6 on intestinal mucosal barrier function. (A) Chemical formulas of Actg2-6. (B) Representative images of differentiated Caco-2 cells after incubation with Actg2-6. (C) Effect of Actg2-6 on intestinal epithelial permeability, as evaluated by the FITC-dextran paracellular permeability assay. (D) Effect of Actg2-6 on intestinal epithelial permeability, as evaluated by the TEER assay. (E) The proteins related with TJ proteins were detected by Western blotting. (F) Representative images of intestinal sections from rats of the sham operated and 1I/6R groups stained with H&E or the IHC marker Ki67 or ZO-1, respectively, and quantified histopathologically based on Chiu’s score or immunoreactive scores, respectively. *P < 0.05; **P < 0.01; ***P < 0.001.