Research Paper Volume 15, Issue 21 pp 12497—12512

Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis

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Figure 6. FA improves the sperm binding ability of aged oocytes. (A) Representative fluorescence images of sperm binding to the surface of the zona pellucida surrounding oocytes stained with Hoechst 33342 from the three groups (Fresh, Aged, and Aged + FA group). Scale bar: 100 μm. R = 3. (B) Number of sperm binding to the surface of the zona pellucida surrounding oocytes from the three groups (Fresh, Aged, and Aged + FA group). *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences.