Research Paper Volume 15, Issue 21 pp 12497—12512
Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis
Figure 2. FA relieves aging-induced oocyte oxidation resistance. (A) Oocytes were stained with DCFH and CMF2HC to detect the intracellular ROS and GSH levels. Scale bar: 100 μm. R = 3. (B, C) Relative intracellular levels of ROS and GSH in bovine oocytes of the three groups (Fresh, Aged and Aged + FA group). (D, E) Relative intracellular activity of CAT and SOD in bovine oocytes from the three groups (Fresh, Aged, and Aged + FA). R = 4. *P < 0.05, **P < 0.01, and ***P < 0.001 indicate significant differences.